Thiohydantoin method
WebThe alkylated-thiohydantoin method for C-terminal sequencing makes a significant improvement to the thiohydantoin method first described by Schlack and Kumpf. Prior to … WebJun 29, 2010 · The method comprises the following steps of: (1) adding the benzil, the thiourea, alkali and a solvent into a reaction kettle; (2) performing a reflux reaction under a normal pressure condition;...
Thiohydantoin method
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WebMar 21, 2024 · Results. The post-thermocycling shear bond strengths of the MDTHP primer were as follows (median, n = 11): 13.2 MPa on silver, 25.9 MPa on copper, 4.1 MPa on palladium, and 11.3 MPa on gold. The MDTHP primer showed higher post-thermocycling shear bond strength on all the four metals. Additionally, on silver and copper, the MDTHP … WebJan 1, 2000 · The alkylated-thiohydantoin method includes the presequencing modification of cysteine and lysine and the automated modification of aspartic and glutamic acids, …
WebA method for the C-terminal sequencing of a peptide in which an alkyl or aryl tin isothiocyanate coupling reagent is used to form a thiohydantoin derivative. Free halogen producing activating... WebEventually, the “thiohydantoin” in the organic phase was hydrolyzed in alkali and the amino acid identified by paper chromatography. A drawback of this method, Edman said, was …
WebThe present invention relates to methods for forming an amino acid thiohydantoin, for determin- ing the C-terminal amino acid of a peptide, for sequencing a peptide from its C-terminal peptide end, and to reagents useful in the methods. References Cromwell, L.D., et al., Biochemistry 8:4735 WebThe density functional theory (DFT) method is utilized to better apprehend the mechanisms of thiohydantoin formation and kinetics through which these processes are facilitated, as well as for studying important intermediates and transition states. ... Kinetics and mechanism of amino acid derived 2-thiohydantoin formation reactions P. Stanić, B ...
WebJan 1, 1996 · A thiohydantoin derivative, THPA, was synthesized according to the method described by Szabo and Karabinos [15] and Duggan et al. [16]. ... Synthesis and characterization of a methacrylate...
WebThe thiohydantoin method has been applied to the stepwise degradation of peptide chains from the carboxyl end. In the present procedure, the thiohydantoin intermediate formed from the peptide, ammonium thiocyanate, and acetic anhydride, was deacylated with barium hydroxide; the thiohydantoin was separated from other substances by means of a double … filenet case foundationWebNov 11, 2014 · In the present work the structural and spectral characteristics of 5-benzyl-2-thiohydantoin (5-BTH) have been studied by methods of infrared, Raman spectroscopy and quantum chemistry. Electrostatic potential surface, optimized geometry, harmonic vibrational frequencies, infrared intensities and acti … filenet bulk import toolWebThe alkylated-thiohydantoin (ATH) method for C-terminal sequence analysis, which exploits some unique properties of the thiohydantoin (TH) ring system, was first described in 1992 … grohe adjustable hand shower holderWebMar 1, 2002 · The Schlack-Kumpf degradation, also called the isothiocyanate method, is thought to be a promising approach to chemical C-terminal sequencing of peptides and … grohe agent bahrainWebThese results suggest that the thiohydantoin method should be useful for the stepwise peptide sequence analysis starting from the carboxyl terminus. The sequence of a model tripeptide was determined by the combination of the thiohydantoin method and mass spectrometry. In the case of peptide which contained a proline residue as their C-terminal ... filenet corporationWebJun 26, 2024 · A solution of trisubstituted thiohydantoin 4 (1 equiv) and sarcosine (2 equiv) in ethanol was refluxed for 30 min, after which the corresponding isatin (2 equiv) was added, and the mixture was refluxed for 6–8 h. The reaction progress was monitored by TLC. grohe adjustable hand shower chrome 100mmWebMar 27, 1990 · Hydrolysis with 2% aqueous triethylamine under mild conditions (1-5 min at 50 degrees C) was found to be more suitable for carboxy-terminal sequence analysis by … grohe adjustable rain shower head