Webagent to the purified enzyme in presence of sodium azide (0.1%) increased the stability of urease up to 15 months (455 days) (Figure 3). The free ammonia content in the purified … WebAlkaline stable (pH 7.75–12.5) urease from Sporosarcina ureae was purified over 400-fold by ion exchange and hydrophobic interaction chromatography. The cytoplasmic enzyme …
Frontiers Diverse Enzymes With Industrial Applications in Four ...
WebThe urease (E.C.3.5.1.5) catalyzes the hydrolysis of urea to ammonia and is commonly used in clinical laboratories for the estimation of urea in biological fluids. The urease enzyme of … WebThe amino acid sequence of jack bean urease has been determined. The protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The subunit relative molecular mass calculated from the sequence is 90770, indicating that urease is composed of six subunits. Out of 25 histidine residues in urease, 13 christ episcopal church ballston spa ny
Characterization of L-asparaginase from - Springer
WebDec 31, 2009 · The results of this study revealed that urease was purified 10.4 fold with a yield of 7.2%. Furthermore, the results of the enzyme characterization experiments proved that urease activity was maximal when pH was 7.5, and optimal activity attained when temperature of incubation was around 40°C. WebHelicobacter pylori urease activity is toxic to human gastric epithelial cells. Infect Immun. 1990 Jun; 58 (6):1992–1994. [PMC free article] [Google Scholar] Solnick JV, O'Rourke J, … WebUrease is a commonly used enzyme in clinical laboratories for the estimation of urea in body fluids such as blood, urine and the like. Urease, with a purity meeting the requirements of … george costanza looking busy gif