WebAug 15, 2005 · Bands at approximately 980 and 1080cm(-1) in solution Raman spectra of phosphoserine and phosphothreonine are assigned to the monobasic and dibasic phosphate groups, respectively. Calibrated Raman peak area ratio measurements, performed as a function of pH, are used to determine the corresponding pKa values of 5.6 … WebJan 2, 2007 · In the case of phosphoserine Ab, blots were blocked for 1 h at room temperature, probed (1:1000) overnight at 4 °C and incubated with HRP-conjugated goat anti-rabbit Ab (1:2000) for 1 h at room temperature. Signal was developed using enhanced chemiluminescence Western blot detection reagents (Pierce).3. Results3.1.

p-Ser/Phosphoserine Antibody (16B4) SCBT - Santa Cruz …

WebPhosphatidylserine is a fatty substance called a phospholipid. It covers and protects the cells in your brain and carries messages between them. Phosphatidylserine plays an … WebIt is a conjugate acid of an O-phosphonato-L-serine (2-). It is an enantiomer of an O-phospho-D-serine. ChEBI. Phosphoserine is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). E. coli Metabolome … st helena live camera

Phosphoserine/threonine Antibody – ECM Biosciences

WebRabbit anti-Phosphoserine Catalog No. 61-8100 Lot No. Rabbit anti-Phosphoserine FORM This antibody is supplied as a 200 µl aliquot at a concentration of 0.25 mg/ml in PBS (pH 7.4) containing 0.1% NaN 3. This polyclonal antibody was purified from rabbit antiserum by phosphoserine-specific, affinity chromatography. WebOct 15, 2004 · FRET would be expected to occur only where BACE contained a phosphoserine epitope. Rabbit anti-BACE CT Ab (1:500; Calbiochem) served as a positive control. Cells were then washed three times in TBS and labeled with Cy3-conjugated anti-mouse Ab or anti-rabbit Ab (10 μg ml –1; Jackson Immunoresearch, West Grove, PA) for 1 … WebFeb 13, 2015 · Immunoblot data showed that the phosphopeptide but not the peptide without the phosphoserine eluted the AKB1 from the co-IP complexes efficiently, ... About 5 µl of each eluates were subjected to WB using anti-AKB1 Ab (left panel) and kinase assay using PKC Pep-Tag assay kit substrate (right panel). ... st helena little league baseball