WebMay 21, 2024 · Hemagglutination inhibition (HI) assay The inhibitory effect of CST on viral attachment was evaluated by the HI inhibition assay [ 28 , 30 ]. Briefly, 50 μl of CST or RBV in serial two-fold dilutions in saline were mixed with an equal volume of influenza virus suspension and incubated for 30 min at 4 °C. A general procedure for HA is as follows, a serial dilution of virus is prepared across the rows in a U or V- bottom shaped 96-well microtiter plate. The most concentrated sample in the first well is often diluted to be 1/5x of the stock, and subsequent wells are typically two-fold dilutions (1/10, 1/20, 1/40, etc.).The final well serves as a negative control with no virus. Each row of the plate typically has a different virus and the same pattern of dilutions. After serial dilutions, a standardi…
Hemagglutination Inhibition PADA SERUM MERPATI …
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WebJun 29, 2024 · The hemagglutination inhibition assay (HAI) is widely used to evaluate vaccine-induced antibody responses as well as to antigenically characterize influenza viruses. The results of an HAI assay are based on an endpoint titration where the titers are generally manually interpreted and recorded by a well-trained expert. For serological … WebMay 19, 2024 · Uses of Hemagglutination-Inhibition Test. Hemagglutination inhibition test is widely used for the diagnosis of infection caused by orthomyxoviruses (influenza), … WebThe hemagglutination inhibition (HI) assay is used to titrate the antibody response to a viral infection. The HI assay takes advantage of some viruses' ability to hemagglutinate (bind) … ramsay multicraft test guide