Cspb promoter
WebDec 31, 2002 · AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal … WebThe xfp gene encoding PKT was cloned from Bifidobacterium animalis and overexpressed under the strong cspB promoter in C. glutamicum. A functional enzyme was detected in an L-Glu-producing strain of C. glutamicum (odhA). When cells of this producer strain with the xfp gene and those without the xfp gene were cultivated in a controlled ...
Cspb promoter
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WebFeb 6, 2012 · The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos). MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. WebJan 16, 2024 · The Duffer Brothers, Brittany Howard, Lyle Lovett, Qui Nguyen, Angie Thomas, Billy Bob Thornton, Mary Steenburgen and Jesmyn Ward Among Featured …
WebA new class of cold shock-induced proteins that may be involved in an adaptive process required for cell viability at low temperatures or may function as antifreeze proteins in … WebApr 18, 2024 · Construction of gadB2 expression strains under different RBS sequence and promoter. The nucleotide sequences of all primers are listed in Table 2.A series of RBS sequence derived from the consensus sequence AGGAG and an AS of 6–8 nt was designed and applied for expressing gadB2.For expressing with normal RBS sequence and AS of …
WebCPB distributes community service grants (CSGs) to noncommercial public television and radio stations that provide significant public service programming to their communities. … WebThe cspB promoter shows sequence similarity to the cspA promoter. Furthermore, both cspA and cspB mRNAs have unusually long 5′ untranslated regions (159 and 161 bases, respectively), both of which are able to form similar extensive secondary structures. These features are considered to contribute to the nature of the thermostats for cspA and cspB
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WebJan 1, 2000 · The cspB promoter (PcspB) described by Peyret et al. (1993) was isolated as a. 554 bp Eco RI \ Eco NI fragment and ligated to an Eco NI \ Bam HI adaptor constructed with the two synthetic oligo- local flower delivery glasgowWebWe used alpha-amylase from Streptococcus bovis 148 (AmyA) as a model protein to be displayed on the C. glutamicum cell surface. AmyA was fused to the C terminus of the porins PorB, PorC, or PorH. Expression vectors using fused proteins under the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. indian coast guard promotionWebcspB promoter was constructed by cross-over PCR as described previously (Kikuchi et al. 2006). First-round PCR amplified the cspB promoter using Primer A (Csp5K, forward primer, Table 2) and Primer B (Csp-PPPg, reverse primer) and pPSPTG11 DNA as a template; the pro-PG gene with its own signal sequence was also amplified using indian coast guard recruitment 2021 applyWebJul 5, 2000 · Csp proteins (CspE, CspA, CspB, and CspC) were used for in vitro transcription assays, as described in A: lane 1, no Csp protein; 1.4 μM Csp for lanes 2, 5, … indian coast guard previous year questionWebOct 26, 2024 · The use of constitutive promoters is advantageous because they do not require expensive reagents for induction or optimized induction conditions (Yim et al., 2013). Constitutive promoters derived from the genome of C. glutamicum, such as P sodA, P gapA, P eftu, and P cspB, are known to have high expression levels indian coast guard ratnagiriWebMay 9, 2009 · We used α-amylase from Streptococcus bovis 148 (AmyA) as a model protein to be displayed on the C. glutamicum cell surface. AmyA was fused to the C terminus of the porins PorB, PorC, or PorH. Expression vectors using fused proteins under the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. indian coast guard psb procedureWebunder the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. Immu-nostaining microscopy and flow cytometric analysis revealed that PorB-AmyA, PorC-AmyA, and PorH-AmyA were displayed on the C. glutamicum cell surface. AmyA activity was only detected in the cell fraction of C. indian coast guard rank